In conclusion, the above have shown us how to use HPLC Ion Exchange Chromatography to determine the amount of ammonia in a whole tobacco. The accuracy of the results depends on the correct use of an HPLC column. After the ammonium ion has eluted, perform gradient elution. This is to remove any divalent cations and left over molecules which may interfere with subsequent samples that are injected. Also, the HPLC should be handled with care to extend the life time of a column.
Since we are using the external standard method, the standards and the sample are measured in chromatography separately. The results of the two chromatogram will then be compared. Thus, chromatograohic conditions must be maintained extremely constant. This problem in this experiment is solved by using the auto-sampler. Auto-sampler injection is more accurate compared to the manual injection.