Tobacco companies discovered that adding ammonia to the cigarette manufacturing process helps convert bound nicotine molecules in tobacco smoke into free nicotine molecules. This process is known as "freebasing." Similar to the chemical process of freebasing cocaine, the end result is an enhanced effect of the drug on the user.
Ion Exchange Chromatography
Ion Exchange Chromatography
Ion Exchange Chromatography is a common method used to purify the proteins and other charged molecules. In cation exchange chromatography positively charged molecules are attracted to a negatively charged solid support. On the other hand, in anion exchange chromatography, negatively charged molecules are attracted to a positively charged solid support.
Cations are positively charged and Anions are negatively charged.
Cations are positively charged and Anions are negatively charged.
Ion Exchange chromatography - Separation conditions
(A) Adsorption of desired substances
(The substance of interest will bind to the exchanger and unwanted substances are eluted out)
(The substance of interest will bind to the exchanger and unwanted substances are eluted out)
(B) Adsorption of unwanted substances
(The unwanted substances will be adsorbed and substance of interest will pass through the column)
Ion Exchange chromatography -Elution types
(The unwanted substances will be adsorbed and substance of interest will pass through the column)
Ion Exchange chromatography -Elution types
1. Isocratic elution
(Elution with single buffer of constant pH and ionic strength)
(Elution with single buffer of constant pH and ionic strength)
2. Stepwise or gradient elution
(The pH or ionic strength of buffer will change in the process of elution)
(The pH or ionic strength of buffer will change in the process of elution)
Posted on: Tuesday, 31 July 2012 10:48 by Su-Lynn and Adam →
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